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human osteosarcoma u 2os cell line  (ATCC)


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    ATCC human osteosarcoma u 2os cell line
    Human Osteosarcoma U 2os Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human osteosarcoma u 2os cell line/product/ATCC
    Average 99 stars, based on 8331 article reviews
    human osteosarcoma u 2os cell line - by Bioz Stars, 2026-06
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    (A) Immunofluorescence micrographs <t>of</t> <t>U-2OS</t> cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. After immunostaining for PML and SUMO2/3 antibodies, the cells were counterstained with DAPI (Nuc). Scale bar = 20 μm. An arrow indicates an EnPB. (B) Western blot analyses for PML and SUMO2/3 in U-2OS cells. The cells were treated with either 0.3 or 3 μM As 3+ for 2 or 72 h, or left untreated, and then lyzed with ice-cold RIPA buffer. The lysate was separated into soluble and insoluble fractions by centrifugation. (C) Western blot analyses for PML, SUMO2/3, SUMO1, and ubiquitin in U-2OS cells. The cells were pretreated with 20 μM ML792 (SUMO E1 inhibitor) or 10 μM TAK294 (ubiquitin E1 inhibitor) for 3 h, and were exposed to 3 μM As 3+ for 2 h in the presence of the inhibitor. (D) Immunofluorescence micrographs of PML -/- U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. Scale bar = 20 μm. (E) Western blot analyses for PML and SUMO2/3 in the soluble fractions of wild U-2OS and PML -/- U-2OS cells. Note that SUMOylated proteins were detected in low electromobility region in both cell types. An open arrowhead indicates SUMO monomers. A right curly bracket indicates PML isoforms.
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    (A) Immunofluorescence micrographs of U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. After immunostaining for PML and SUMO2/3 antibodies, the cells were counterstained with DAPI (Nuc). Scale bar = 20 μm. An arrow indicates an EnPB. (B) Western blot analyses for PML and SUMO2/3 in U-2OS cells. The cells were treated with either 0.3 or 3 μM As 3+ for 2 or 72 h, or left untreated, and then lyzed with ice-cold RIPA buffer. The lysate was separated into soluble and insoluble fractions by centrifugation. (C) Western blot analyses for PML, SUMO2/3, SUMO1, and ubiquitin in U-2OS cells. The cells were pretreated with 20 μM ML792 (SUMO E1 inhibitor) or 10 μM TAK294 (ubiquitin E1 inhibitor) for 3 h, and were exposed to 3 μM As 3+ for 2 h in the presence of the inhibitor. (D) Immunofluorescence micrographs of PML -/- U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. Scale bar = 20 μm. (E) Western blot analyses for PML and SUMO2/3 in the soluble fractions of wild U-2OS and PML -/- U-2OS cells. Note that SUMOylated proteins were detected in low electromobility region in both cell types. An open arrowhead indicates SUMO monomers. A right curly bracket indicates PML isoforms.

    Journal: bioRxiv

    Article Title: Biological differences in promyelocytic leukemia (PML) proteins between PML-nuclear bodies (PML-NBs) and extranuclear PML bodies (EnPBs) in arsenite-exposed cells

    doi: 10.64898/2026.01.25.701640

    Figure Lengend Snippet: (A) Immunofluorescence micrographs of U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. After immunostaining for PML and SUMO2/3 antibodies, the cells were counterstained with DAPI (Nuc). Scale bar = 20 μm. An arrow indicates an EnPB. (B) Western blot analyses for PML and SUMO2/3 in U-2OS cells. The cells were treated with either 0.3 or 3 μM As 3+ for 2 or 72 h, or left untreated, and then lyzed with ice-cold RIPA buffer. The lysate was separated into soluble and insoluble fractions by centrifugation. (C) Western blot analyses for PML, SUMO2/3, SUMO1, and ubiquitin in U-2OS cells. The cells were pretreated with 20 μM ML792 (SUMO E1 inhibitor) or 10 μM TAK294 (ubiquitin E1 inhibitor) for 3 h, and were exposed to 3 μM As 3+ for 2 h in the presence of the inhibitor. (D) Immunofluorescence micrographs of PML -/- U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. Scale bar = 20 μm. (E) Western blot analyses for PML and SUMO2/3 in the soluble fractions of wild U-2OS and PML -/- U-2OS cells. Note that SUMOylated proteins were detected in low electromobility region in both cell types. An open arrowhead indicates SUMO monomers. A right curly bracket indicates PML isoforms.

    Article Snippet: U-2OS cells (HTB-96) were obtained from ATCC and cultured in McCoy’s 5A medium containing 10% heat-inactivated fetal bovine serum (FBS).

    Techniques: Immunofluorescence, Immunostaining, Western Blot, Centrifugation, Ubiquitin Proteomics

    (A) Immunofluorescent micrographs of PML(VI) U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. Scale bar = 20 μm. An arrow indicates an EnPB. (B) Numbers of PML-NBs and EnPBs in untreated and As 3+ -exposed PML(VI) U-2OS cells. An agglomeration of three or more than three PML blobs in the perinuclear region was counted as an EnPB. More than 80 cells were observed for counting PML-NBs and EnPBs. Data are presented as mean ± SD. *, Significantly different from the untreated cells. (C) Western blot analysis for PML and SUMO2/3 following exposure to 3 μM As 3+ for 0, 2, 8, 24, 48, or 72 h in PML(VI) U-2OS cells. Closed and open arrowheads indicate PML-VI and SUMO2/3 monomers, respectively. A right square bracket indicates SUMOylated PML-VI.

    Journal: bioRxiv

    Article Title: Biological differences in promyelocytic leukemia (PML) proteins between PML-nuclear bodies (PML-NBs) and extranuclear PML bodies (EnPBs) in arsenite-exposed cells

    doi: 10.64898/2026.01.25.701640

    Figure Lengend Snippet: (A) Immunofluorescent micrographs of PML(VI) U-2OS cells. The cells were exposed to 3 μM As 3+ for 2 or 72 h, or left untreated. Scale bar = 20 μm. An arrow indicates an EnPB. (B) Numbers of PML-NBs and EnPBs in untreated and As 3+ -exposed PML(VI) U-2OS cells. An agglomeration of three or more than three PML blobs in the perinuclear region was counted as an EnPB. More than 80 cells were observed for counting PML-NBs and EnPBs. Data are presented as mean ± SD. *, Significantly different from the untreated cells. (C) Western blot analysis for PML and SUMO2/3 following exposure to 3 μM As 3+ for 0, 2, 8, 24, 48, or 72 h in PML(VI) U-2OS cells. Closed and open arrowheads indicate PML-VI and SUMO2/3 monomers, respectively. A right square bracket indicates SUMOylated PML-VI.

    Article Snippet: U-2OS cells (HTB-96) were obtained from ATCC and cultured in McCoy’s 5A medium containing 10% heat-inactivated fetal bovine serum (FBS).

    Techniques: Western Blot